Table 1. Strains.
| Strains | Description | Reference | |
|---|---|---|---|
|
E. coli strains |
DH10B |
Standard laboratory cloning strain |
Invitrogen |
| |
Rosetta(DE3)pLysS |
E. coli expression strain |
Novagen |
|
S. aureus strains |
SA564 |
S. aureus clinical isolate |
43
|
| |
SA564 cshA::tt |
SA564 cshA::Ll.LtrB group II intron after nucleotide 97 |
This study |
| |
S30 |
S. aureus clinical isolate |
23
|
| |
S30 agrA::tt |
S30 having agrA disrupted by group II intron after nucleotide 442 |
This study |
| |
S30 cshA::Mu |
S30 cshA::mini-Mu, clone Ω4536 |
23
|
| |
Ω4536-C |
S30 cshA::mini-Mu, clone Ω4536 complemented with CshA |
23
|
| |
S30 cshA::Mu agrA::tt |
S30 cshA::mini-Mu agrA::tt group II intron after nucleotide 442 |
This study |
| |
PR01 |
SA564 disrupted for two restriction systems and deleted pyrFE |
61
|
| PR01ΔcshA | PR01 with cshA deleted | This study |