pMK4Xyl
|
E. coli-S. aureus shuttle vector pMK4 with a xylose-inducible promoter
|
23
|
pMK4gfp
|
pMK4Xyl with gfp
|
23
|
pMK4cshA
|
pMK4Xyl with cshA from S30
|
23
|
pMK4cshA-K52A
|
pMK4Xyl with cshA-K52A
|
This study
|
pNL9162
|
pT181 based plasmid Ll.LtrB group II intron targetron
|
42
|
pNL9162-cshA
|
pNL9162 modified to insert the intron into cshA (anti-sense)
|
This study
|
pMK4-targetron-agrA
|
Ll.LtrB group II targetron in pMK4 for disruption of agrA
|
This study
|
pCN47
|
pT181 based shuttle vector with an Erm cassette
|
62
|
pCN38
|
pT181 based shuttle vector with a cat194 cassette
|
62
|
pEB01
|
pCN47 with the cat194 cassette from pCN38 replacing the Erm cassette
|
This study
|
pEB07
|
pEB01 with cshA gene from SA564 and 542 bp upstream, cloned between the BamHI and the SmaI-site
|
This study
|
pET15b
|
E. coli expression plasmid for purification of proteins with N-terminal His6-tag
|
Novagen
|
pET22b
|
E. coli expression plasmid for purification of proteins with C-terminal His6-tag
|
Novagen
|
pET22b-cshA
|
cshA cloned in NdeI and BamHI sites
|
This study
|
pET15b-RNase J1
|
RNase J1 gene cloned into NdeI and BamHi sites
|
This study
|
| pET15b-RNase J2 |
RNase J2 gene cloned into NdeI and BamHi sites |
This study |
