Figure 2. The classical autophagy pathway is required for IFN1@-induced autophagy. (A) K562 cells were transfected with indicated shRNA for 48 h, and then the mRNA and protein expression of these shRNA targeted genes were analyzed by real-time PCR and western blot, respectively. (B) K562 cells were transfected with indicated shRNA for 48 h and then treated with IFN1@ (1000 U/ml) for 48 h. Autophagy was assayed by western blot analysis of LC3 expression or quantitation of average number of LC3 puncta per cell (B). *p < 0.05 vs. control shRNA group.