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. 2013 Mar 6;8(3):e57968. doi: 10.1371/journal.pone.0057968

Figure 1. 14-3-3ε induces cell migration.

Figure 1

(A) Endogenous level of 14-3-3ε was determined in HCC cell lines including Huh-7, SK-Hep1, HepG2, Hep3B and PLC-5 cells by Western blotting analysis. (B) 14-3-3ε overexpression stable cells were confirmed by Western blotting analysis. Control, stable clones of empty vector (p3XFlag); 14-3-3ε, stable clones of p3XFlag-14-3-3ε. (C) Cell migratory abilities of 14-3-3ε overexpression and control cells were determined by Boyden chamber assay. (D) Effect of cell migration by transient and dose-dependent transfection of 14-3-3ε in Huh-7 and HepG2 cells. (E) Cells transfected with scramble or 14-3-3ε siRNA were subjected to detect the reduction of 14-3-3ε expression (upper panel) by Western blotting and cell migration (lower panel). Knockdown of 14-3-3ε with siRNA significantly inhibited 14-3-3ε-induced cell migration. (F) Knockdown of 14-3-3ε inhibited cell migration with a dose-dependently manner in SK-Hep1 cells. These data are from three independent experiments. Scale bars: mean ± SD. *P<0.05, **P<0.01.