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. 2013 Mar 6;8(3):e57968. doi: 10.1371/journal.pone.0057968

Figure 3. 14-3-3ε induces Zeb-1 and Snail expression.

Figure 3

(A) Western blotting analysis of Zeb-1, Zeb-2, Snail, Twist, and Slug expression in control and 14-3-3ε overexpression cells. Actin was used as loading control. (B) Quantitative real-time PCR analysis of Zeb-1 and Snail expression in control and 14-3-3ε overexpression cells. Scale bars: mean ± SD. *P<0.05, **P<0.01. (C) Effects of Zeb-1, Snail and E-cadherin expression by transient and dose-dependent transfection of 14-3-3 were analyzed by Western blotting analysis. Actin was used as loading control. (D) 14-3-3ε-induced Snail and Zeb-1 expression was suppressed by 14-3-3ε siRNA knockdown compared with scramble siRNA. Actin was used as loading control. (E) Cells were transfected with scramble, Snail or Zeb-1 siRNAs for 48 hours and cell migration was determined by Boyden chamber assay. 14-3-3ε–induced cell migration was abrogated by Snail or Zeb-1 siRNA knockdown. These data are from three independent experiments. Scale bars: mean ± SD. *P<0.05, **P<0.01.