Table 2.
Semi-quantitative gene expression 0 to 4+ by visual estimation in human podocyte, human foreskin fibroblast cell line (HFF) and human monocytic cell line (THP-1) for Toll like receptor (TLR) TLR1 to TLR10, and cofactors MD-1 and MD-2 on reverse transcription polymerase chain reaction (RT-PCR). Podocytes were also tested with and without 10 % fetal bovine serum in media
| Podocyte (serum free) | Podocyte (with serum) | HFF | THP-1 | |
|---|---|---|---|---|
| TLR1 | 2+ | 3+ | 2+ | 3+ |
| TLR2 | 2+ | 2+ | 2+ | 3+ |
| TLR3 | 2+ | 2+ | 3+ | 2+ |
| TLR4 | 2+ | 3+ | 4+ | 4+ |
| TLR5 | 2+ | 3+ | 3+ | 3+ |
| TLR6 | 3+ | 3+ | 4+ | 4+ |
| TLR7 | 0 | 0 | 0 | 3+ |
| TLR8 | 0 | 0 | 0 | 3+ |
| TLR9 | 0 | 0 | 0 | 3+ |
| TLR10 | 2+ | 2+ | 3+ | 3+ |
| MD-1 | 0 | 0 | 0 | 3+ |
| MD-2 | 3+ | 3+ | 4+ | 4+ |