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. 2013 Feb 25;3(3):201–209. doi: 10.7150/thno.5743

Figure 3.

Figure 3

Influence of PEG MW on siRNA blood clearance and organ distribution in mice. Northern blot was performed for analysis of siLNA, PEG5k-siLNA, PEG10k-siLNA and PEG20k-siLNA after i.v. injection of 200 μl in mice (n=2). (A), siRNA stability in blood circulation: The main band, corresponding to intact siRNA, was harvested at the indicated time points post injection. Loading order: lanes 1-3 controls, (C, C1 and C2) correspond to 1, 0.5 and 0.1 ng unformulated siRNA, respectively, for all 4 blots; lanes 3-7 and 8-12, RNA samples from blood harvested 1, 15, 30, 60 and 120 min post injection from mouse 1 and 2 in each group, respectively. (B). siRNA biodistribution: Lane 1, 0.1 ng siRNA (Control); lanes 2-5 and 6-9 are samples obtained from organs (Lu-lung, Sp-spleen, Ki-kidney and Li-liver) from mouse 1 and 2 at 2 h, respectively; two blots represented mice injected with siLNA/PEG5k-siLNA, and PEG10k-siLNA/PEG20k-siLNA, respectively. (C). PEG20k-siLNA biodistribution 24 h postinjection: Lane 1, 0.1 ng siRNA (Control); lanes 2-5, 6-9, 10-13 and 14-17 are samples from organs (lung, spleen, kidney and liver) from mouse 1 - 4, respectively, and the same order of loading was performed for mouse 5 and mouse 6 which injected with siLNA alone.