Figure 4.

shows the immunocytochemical images of one representative clinical sample of a Lyme disease patient whose diagnosis was supported by the 2-tiered serological method that follows the CDC surveillance testing guidelines. The serum sample was cultured with our culture method for 8 weeks and the obtained collagen-coated slides were immunostained with polyclonal fluorescent antibodies against Borrelia burgdorferi. Panels A-D represent 4 different fields of one collagen-coated slide showing individual spirochetes and small and large spirochetal aggregates that stained positively for Borrelia burgdorferi antigen (green staining). Magnification: 200X.