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demonstrates immunocytochemical images of nine representative positive clinical samples of the 72 Lyme disease patients whose diagnosis was supported by the 2-tiered serological method that follows the CDC surveillance testing guidelines. The samples were cultured with our novel method for 8 weeks and immunostained with polyclonal fluorescent antibodies against Borrelia burgdorferi (green staining). Panels A-I: Nine representative samples, 400x.
