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Representative analysis of chromosomal DNA for exogenous gene (GFP) insertion for three offspring (R1–R3). DNA isolated from blood samples were analyzed for GFP gene by PCR amplification (a) and southern blot hybridization (b). Gene copy number of GFP and Sox2 in genome was estimated to be same by Real-Time PCR (c). The GFP gene in chromosomal DNA of rat supported the evidence for transdifferentiation of MSCs into functional sperms.
