Table 1.
Immunocytochemical properties of rAT-MSCs.
| Antibody/marker | Dilution | Source | Detection |
|---|---|---|---|
| CD 26 | 1 : 50 | Santa Cruz Bio. | + |
| CD 34 (C-18) | 1 : 150 | Santa Cruz Bio. | ∅ |
| CD 45 (H-230) | 1 : 150 | Santa Cruz Bio. | ∅ |
| CD 71 (K-20) | 1 : 150 | Santa Cruz Bio. | ∅ |
| CD105/Endoglin (M-20) | 1 : 100 | Santa Cruz Bio. | + |
| c-Fos (4) | 1 : 50 | Santa Cruz Bio. | + |
| Collagen II (2B1.5) | Predilute | Thermo Scientific | + |
| Collagen Ia1 (D-13) | 1 : 50 | Santa Cruz Bio. | + |
| β-tubulin | 1 : 50 | Santa Cruz Bio. | + |
| Nestin (Rat-401) | 1 : 50 | Santa Cruz Bio. | + |
| Vimentin (C-20) | 1 : 100 | Santa Cruz Bio. | + |
| Fibronectin (EP5) | 1 : 100 | Santa Cruz Bio. | + |
| ASMA | 1 : 800 | Thermo Scientific | + |
| Myogenin (F5D) | Predilute | Thermo Scientific | + |
| MAP 2a, b (AP20) | Predilute | Thermo Scientific | + |
| GFAP | Predilute | Thermo Scientific | + |
| Osteocalcin (FL-100) | 1 : 50 | Santa Cruz Bio. | + |
| Osteonectin (SPARC) | 1 : 50 | Millipore | + |
| Osteopontin (AKm2A1) | 1 : 50 | Santa Cruz Bio. | + |
| Ki67 | 1 : 300 | Thermo Scientific | + |
| PCNA | 1 : 200 | Thermo Scientific | + |
| Tenascin-C | 1 : 50 | Santa Cruz Bio. | + |
| Cytokeratin 18 | 1 : 50 | Santa Cruz Bio. | ∅ |
+: positive; ∅: lack of marker expression.