Figure 1.

Silencing the YB-1 gene reduces cell proliferation and downregulates YB-1 expression at the protein level in OS cell lines. (A) Growth curve of MG63 and MNNG cells transfected with si-Ctr or si-YB-1#1 monitored up to 96-h post-transfection. Experiments were performed in triplicate, and data are expressed as the mean±s.d. *P<0.05. (B and C) Downregulation of YB-1 protein in MG63 and MNNG cells as shown by western blotting after silencing of the YB-1 gene. Actin was used for internal normalisation. Relative intensity is shown under each blot. si-YB-1#1 treatment resulted in downregulation of YB-1 protein expression in a dose-dependent (B) and time-dependent (C) manner.