Figure 2.

Effects of si-YB-1 on cell cycle progression in human OS cell lines. (A) Representative cell cycle profile of MG63 and MNNG cells transfected with si-Ctr, si-YB-1#1, or si-YB-1#2. Cells were treated with siRNA for 72 h, and then detached from the substratum by limited trypsin digestion, and a single-cell suspension was used for propidium iodide staining. DNA content in single cells was measured by flow cytometry. (B) Increased G1/G0 and decreased S phase DNA content by YB-1 knockdown in MG63 and MNNG cell lines. Experiments were performed in triplicate, and data are expressed as the mean±s.d. *P<0.05.