Figure 6.

Cytarabine treatment in MMR-deficient cells is associated with an increase in ROS in a dose-dependent manner. Quantification of ROS in HCT116 and HCT116+Chr3 cells exposed to cytarabine or control for different durations. Samples were taken from homogenised cells (A) and growth media (B). The resulting fluorescent signal was quantified using a 96-well plate reader. Assays were performed in triplicate. Error bars represent the s.e.m. Statistical values were obtained using a two-tailed t-test.