Figure 4.

Survivin levels remain suppressed in in vivo xenografts in nude mice where OS remains elevated. HMLE ^HRAS cells (5 × 10⁶ cells) were subcutaneously injected in nude mice and the xenografts grown for various time points were subjected to (A) 8-iso-PGF2α analysis or (B) MDA+ HNE analysis. (C) Top panel: pictographs of xenografts obtained after various time points. Bottom panel: NOX1, p67, survivin, pERK1/2, cleaved caspase-3 and GAPDH analysis by western blot. (D) Overexpression of control vector (Con) or full-length survivin (Surv OE) in HMLE ^HRAS cells and analysis of NOX1 protein expression by western blot analysis (E) Control and Surv OE cells were subcutaneously injected in nude mice. Tumour volumes were analysed after various time points. (F) MDA-MB-231 and MDA-MB-468 breast cancer (5 × 10⁶) cells were injected in nude mice and the xenografts grown for various time points were subjected to 8-iso-PGF2α analysis and (G) NOX1, NOX4, survivin, MKP-1 and GAPDH protein expression by western blot analysis. *P⩽0.05, **P⩽0.01 and ***P⩽0.001. Results are representative of three different experiments.