Figure 4.

Increased EpCAM expression levels correlate with cell growth inhibition. SKOV3 cells were transduced with pMX and ZF_BVP64. At day 4 after transduction, cells were equally replated. Next day, siRNA transfection was performed, and subsequently at 3 days, EpCAM protein (A) and 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (B) were analysed. (C) SKOV3 cells were transduced with a constant amount of ZF_BVP64 virus supernatant mixed with pMX and/or ZF_ASKD. At day 4 after transduction, EpCAM protein expression was measured (inset) and transduced cells were replated at equal cell numbers, and MTT assay was performed for the four following days.