Figure 5.

Induction of EpCAM expression in EpCAM-negative ovarian cancer cell line. A2780 cells were transduced to express the indicated ATFs in 6- and 96-wellsplates. (A) At day 4, transduced cells were fixed and stained, or 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT assay was performed at days 4, 5 and 6 after transduction. (B) Transduced cells were harvested at day 4 after transduction, replated at equal cell numbers in 96-well plates and MTT assay was performed for the four following days. OD, optical density.