Table 1.
PCR primers used to detect CagA, CagE, CagM, CagT, orf10, orf13, TnpA, and TnpB in H. pylori isolates.
| Genes | Primers | Nucleotide sequences (5′–3′) | Length of PCR products | Location in genome of reference† |
|---|---|---|---|---|
| cagA | cagA-F | GATAACAGGCAAGCTTTTGAGG | 349 | |
| cagA-R | CTGCAAAAGATTGTTTGGCAGA | 14994–15342 | ||
| cagE | cagE-F | GTTACATCAAAAATAAAAGGAAGCG | 735 | |
| cagE-R | CAATAATTTTGAAGAGTTTCAAAGC | 12342–13076 | ||
| cagM | cagM-F | ACAAATACAAAAAAGAAAAAGAGGC | 587 | |
| cagM-R | ATTTTTCAACAAGTTAGAAAAAGCC | 4815–9264 | ||
| cagT | cagT-F | TCTAAAAAGATTACGCTCATAGGCG | 490 | |
| cagT-R | CTTTGGCTTGCATGTTCAAGTTGCC | 1182–1671 | ||
| orf10 | orf10-F | AATAGTGCTTTCTTTAGGATTAGCG | 658 | |
| orf10-R | CCGATTTAATCCTTTCGCTTATGTG | 8970–9627 | ||
| orf13 | orf13-F | CGTTCATGTTCCATACATCTTTGGC | 617 | |
| orf13-R | GATTTATAGCGATCTAAGAAACCGC | 704–1320 | ||
| tnpA | tnpA-F | ATCAGTCCAAAAAGTTTTTTCTTTCC | 338 | |
| tnpA-R | TAAGGGGGTATATTTCAACCAACCG | 154–541 | ||
| tnpB | tnpB-F | CGCTCTCCCTAAATTCAAAGAGGGC | 578 | |
| tnpB-R | AGCTAGGGAAAAATCTGTCTATGCC | 954–1531 |
†GenBank accession number. AF282853.1.