Figure 2.

BP-IgG–induced GFP-BP180 internalization in live 804G cells and NHEKs. Live cell imaging studies of subconfluent (A) and confluent (C) cultures of 804G cells and subconfluent (B) and confluent (D) cultures of NHEKs. GFP-BP180–expressing cells were visualized by confocal microscopy, either along their substrate-attached surface (basal level) or at a higher focal plane (equatorial level), at various intervals after BP-IgG treatment. In both cell types and conditions, GFP-BP180 was internalized and redistributed centripetally toward the perinuclear region (A–D). Z-sections of the cells are shown at the bottom of each column. At the right, images of the cell border at time 0 and the end of the observation are shown in green and red, respectively. In 804G cells, rounding up of the cells was observed (A and C). Scale bars: 10 μm.