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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Methods Mol Biol. 2011;720:279–292. doi: 10.1007/978-1-61779-034-8_17
40 ml Step 1 + 40 ml Step 9 = 80 ml Step 5
20 ml Step 1 + 20 ml Step 5 = 40 ml Step 3
10 ml Step 1 + 10 ml Step 3 = 20 ml Step 2
10 ml Step 3 + 10 ml Step 5 = 20 ml Step 4
20 ml Step 5 + 20 ml Step 9 = 40 ml Step 7
10 ml Step 5 + 10 ml Step 7 = 20 ml Step 6
10 ml Step 7 + 10 ml Step 9 = 20 ml Step 8