| pGEM-ODC | 10 μg |
| BamHI (Invitrogen) | 10 U |
| EcoRI (Invitrogen) | 10 U |
| 100 × BSA (Invitrogen) | 1 μl |
| Invitrogen React 2 Buffer | 10 μl |
| Nuclease-free water | to a final volume of 100 μl |
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