Figure 4a.

ESR spectrum of 2-chloroaniline when oxidized by HRP/H₂O₂. 2-chloroaniline, MNP, and H₂O₂ were mixed in Chelex-100 treated phosphate buffer and the reaction was initiated by adding HRP. The reaction was transferred to a flat cell and placed in the ESR cavity for recording. The spectrum for 2-chloroaniline was not well resolved, which required specific parameters to be used (see Materials and Methods). The simulation of this spectrum (correlation: r=0.99) is shown below the experimental spectrum (top), and the center peak is zoomed to show the overlay. The hyperfine splitting constants are shown in Table 5.