Fig. 2.

Internal telomeric repeats associate with TRF1 and other shelterin proteins. a Quantification of the recovery of the 2q14 fusions site (2q14 locus) and Rap1 control region (Rap1 gene) in the ChIPs using the indicated antibodies or pre-immune serum (PI) by qPCR in BJ/hTERT/SV40 cells. Ct values collected from each qPCR analysis performed in triplicate were normalized to H3. Ct values were than corrected for the averaged Ct values obtained with the pre-immune antibodies that yielded background signals (PI TRF1, PI TRF2, PI TIN2). Bars represent mean values of three independent experiments with SD. Brackets with asterisks indicate p values below 0.05 (Student’s t test). b Dot blots monitoring the specificity of ChIP with antibodies to shelterin proteins and control sera by hybridization with a TTAGGG repeats probe