Fig. 4.

TRF1 and BLM affect stability of the common fragile site at 2q14. a Immunoblot monitoring shRNA-mediated repression of BLM and TRF1 (day 2 post-infection). γ-Tubulin was used as loading control. b Example of SCEs in control (left panel) and shBLM-treated cells (right panel) and c quantification of SCEs after BLM loss. d Frequency of fragile telomeres after repression of BLM, TRF1, or both. e Quantification of the breakage at 2q14 and f frequency of breakage at NFRA, FRA3B, FRA7H, and FRA16D after repression of BLM, TRF1, or both with or without aphidicolin treatment. In all panels, bars represent mean values of three independent experiments with SD (see Suppl. Tables 3 and 4). Brackets with asterisks indicate p values below 0.05 (Student’s t test)