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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Cell Rep. 2012 Oct 19;2(4):951–963. doi: 10.1016/j.celrep.2012.09.016

Figure 3. CD133 Promotes Cell Proliferation and Tumorigenesis.

Figure 3

(A) Cell number relative to day 0 for Caco-2 and OVCAR-8 cells treated with control, CD133, or HDAC6 shRNAs. Error bars represent SD (n = 3).

(B) Clonogenic assay of Caco-2 and OVCAR-8 cells treated with control, CD133, or HDAC6 shRNAs. Error bars represent SD (n = 3).

(C) Soft agar colony forming assay to measure anchorage-independent growth of Caco-2 and OVCAR-8 cells treated with control, CD133, or HDAC6 shRNAs. Error bars represent SD (n = 3). *p < 0.05 and #p < 0.01 as determined by a Student’s t test.

(D) Tumor volume 40 days after 5 × 105 shRNA-treated OVCAR-8 cells were subcutaneously implanted in NOD/SCID/IL2Rγ−/− mice. Errors bars represent SEM (n = 10). ***p < 0.001 as determined by analysis of variance (ANOVA).

See also Figure S4.