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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Cell Rep. 2012 Oct 19;2(4):951–963. doi: 10.1016/j.celrep.2012.09.016

Figure 4. CD133 Suppresses Cancer Cell Differentiation.

Figure 4

(A) Relative transcript levels of epithelial differentiation markers CK20, MUC2, and FABP2 as measured by quantitative PCR of shRNA-treated Caco-2 cells. Error bars represent SD (n = 3).

(B) Activity of the enterocytic marker alkaline phosphatase (AP) following knockdown of HDAC6 or CD133 in Caco-2 cells. Error bars represent SD (n = 4).

(C) Relative transcript levels of the ovarian differentiation markerHMGA2 as measured by quantitative PCR of shRNA-treated OVCAR-8 cells. Error bars represent SD (n = 3).

(D) Western blot analyses for epithelial (E-cadherin) and mesenchymal (N-cadherin and vimentin) markers in OVCAR-8 cells where CD133 or HDAC6 has been knocked down with two independent shRNAs. CD133 and HDAC6 knockdowns are shown in Figure 4D. GAPDH immunoblots served as a loading control.

(E) Quantitative PCR of EMT-associated and Wnt signaling target genes following knockdown ofCD133orHDAC6inOVCAR-8cells. Error bars represent SD(n= 3).

(F) Invasion of basement membrane extract (BME) of OVCAR-8 cells (24 hr assay) treated with shRNAs to knock down CD133 or HDAC6. Cell invasion values are relative to cell invasion of OVCAR-8 cells treated with the control shRNA targeting a nonhuman transcript, LacZ. Error bars represent SD (n = 3). *p < 0.05 and #p < 0.01 as determined by a Student’s t test.

See also Figure S5.