FIG. 3.

Changes in mRNA identifiability as a function of transcript fragment/read length. Starting from levels achieved with 50-bp single-end reads, the left side of the y-axis shows the additional number of genes that become identifiable using MultiSplice as the read length increases. The y-axis on the right side shows the total percentage of genes for which mRNA transcript structures are resolved. The UCSC annotated transcript sets of four species: human, mouse, fly, and worm were used for this analysis.