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(a) Engrailed-2 (EN-2) promoter methylation status in 13 case and 13 control cerebellum samples using the McrBC-PCR restriction assay; target CpG sites for restriction within the 5′-promoter sequence are highlighted. (b) EN-2 promoter methylation status using methylation-sensitive restriction PCR with HpaII/BstUI restriction enzymes; target CpG sites within the 5′-promoter sequence are highlighted.
