Figure 2.

Selection of p53-deficient fibroblasts in vivo at increasing numbers of tumor-initiating A549 lung cancer cells. Allele-specific PCR analysis for wild-type and p53 mutant alleles indicates a higher prevalence of p53KO fibroblasts at higher numbers of A549 cells. The source of the DNA template for the PCR analysis is indicated above the microphotograph of the gel, while the experimental design is shown below the gel microphotograph. When A549 cells cultured in vitro were used as a template, no PCR product was obtained confirming the specificity of the allele-specific PCR for mouse p53.The result of 1 of 3 representative independent experiments is shown.