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The levels of the factors used to optimize the degree of hydrolysis.
| Code | pH (X 1) | Temperature (X 2, °C) | E/S (X 3) | Incubation period (X 4, h) |
|---|---|---|---|---|
| −2 | 6.00 | 40.00 | 0.06 | 6.00 |
| −1 | 6.50 | 45.00 | 0.08 | 8.00 |
| 0 | 7.00 | 50.00 | 0.10 | 10.00 |
| 1 | 7.50 | 55.00 | 0.11 | 12.00 |
| 2 | 8.00 | 60.00 | 0.13 | 14.00 |
