Figure 4.

(a) Phospholipase activity (indirect hemolysis) of the isolated enzymes from Panama B. asper venom. Samples: pMTX-I (5 μg); pMTX-II (10 μg); pMTX-III (5 μg); pMTX-IV (10 μg). Negative control (PBS) and positive control (VBasp, B. asper venom, 10 μg); (b) myotoxic activity of the PLA₂s isolated from Panama B. asper venom. Swiss mice were injected with 50 μL of the different samples in the right gastrocnemius muscle and, after 3 h, the blood from the tail was collected in capillaries with heparine. Negative (PBS), positive control (BaspV, B. asper venom, 25 μg), and isolated myotoxins (50 μg) were used. Values represent the mean ± S.D. from 3 independent experiments.