Figure 1.

Short-circuit current (I _sc) in permeabilized MDCK cells: Cl⁻-containing media. (a) A typical record is shown to demonstrate the experimental protocol. After equilibration, amphotericin B (10 μM) was added to the apical medium, to permeabilize the apical plasma membrane to small cations. This maneuver allows measuring basolateral pump currents. Then, the effects of ouabain (1 mM) and rotenone (10 μM) were evaluated. (b) Effect of ouabain (1 mM) and rotenone (10 μM) on I _sc of permeabilized MDCK cells. Results are mean ± SEM of five independent experiments.