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. 2012 Dec 23;2013:868131. doi: 10.1155/2013/868131

Table 2.

Primers for site-directed mutagenesis of construct (−1243/+45) p55PIK.

Name Sequence change Sense primer sequence (5′-3′)/antisense primer sequence (5′-3′)
RUNX1-mut ACCACC to GTGTAT GCAACCGGCCTCAGCCAATCGTGTATCACCTTGCCTCTCTTCCCTC
GAGGGAAGAGAGGCAAGGTGATACACGATTGGCTGAGGCCGGTTGC
MZF1-mut1 TGGGGA to CTAGTG GAAGCCTAGAGAGCGGCTAGTGGACGTGAAAGTCGAG
CTCGACTTTCACGTCCACTAGCCGCTCTCTAGGCTTC
MZF1-mut2 GGGGG to TAGTC CGTACCATTCTGATTGAGGCTTAGTCAGAGCGCGGATGGCTGATTGGG
CCCAATCAGCCATCCGCGCTCTGACTAAGCCTCAATCAGAATGGTACG
MZF1-mut3 TGGGGA to CTAGTG CGAGAGGCGTGGCTGATCTAGTGGCCGAAGCCTAGAGAGCGG
CCGCTCTCTAGGCTTCGGCCACTAGATCAGCCACGCCTCTCG
YY1-mut ACCAT to GTTGA CCAGCGCCCGGATCGTGTTGATCTGATTGAGGCTGG
CCAGCCTCAATCAGATCAACACGATCCGGGCGCTGG