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. 2012 Dec 27;2013:918136. doi: 10.1155/2013/918136

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Copyright © 2013 X.-Z. Fu and J.-H. Liu.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Validation of microarray results by semiquantitative RT-PCR. Ten probes putatively encoding lipid transfer protein (Cit.60.1.S1_at), glutathione s-transferase (Cit.6308.1.S1_at, Cit.9510.1.S1_s_at), ABC transporter ATPase (Cit.3246.1.S1_at), phospholipase d (Cit.6097.1.S1_s_at), cell wall invertase (Cit.5734.1.S1_at), miraculin-like protein 2 (Cit.57.1.S1_at) and unknown protein (Cit.19313.1.S1_at, Cit.5367.1.S1_at, Cit.1406.1.S1_s_at) were amplified with specific primers in WT and TG9 leaves, using Actin gene as an internal control for examining equal cDNA loading. The expression ratios between TG9 and WT were calculated by quantifying the band density using the Quantity One software.