Figure 3.

SACT effect of RB on S. aureus. Cells at initial concentration of 10⁶ CFU mL⁻¹ were incubated with 0–5 μM RB in an ultrasonic bath for 1 h in the dark. Controls: not sonicated bacterial cultures with or without PS. After the treatment, bacterial samples were serially diluted in 10-fold dilutions and evenly spread over BHA plates with a Drigalsky spreader. Plates were incubated at 37°C overnight and CFU were counted.