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The expression profiles of germ cell-associated genes during EB formation from iPS cells. Real-time PCR was performed using cDNA from EB cultures maintained for 0, 4, 7, and 14 days (D0, D4, D7, and D14) to detect the relative mRNA expression of Dppa3, Stra8, Tex14, Dazl, Scp1, Scp3, Msy2, and Akap3. The mean normalized expression of each gene relative to that of Gapdh was shown.
