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Expression of male germ cell markers in EBs derived from iPS cells. Expression of MVH (a), CDH1 (d), and SCP3 (g) in the EBs after cultured for 4–7 days was detected by immunofluorescent staining. DAPI (b, e, h) was used to indicate cell nuclei, and merged pictures (c, f, i) were shown. Arrows indicated the cells that were positive for MVH, CDH1, or SCP3. Scale bars = 50 μm.
