Figure 5.

(a) PLA₂ activity of Bothriopsis bilineata venom and peak 7 (Bbil-TX); (b) the inhibitory effect of the antihemorrhagic factor DAII-2 and the crotapotin F3 on PLA₂ activity Bbil-TX; (c) effect of temperature on the PLA₂ activity of Bbil-TX; (d) effect of pH on Bbil-TX activity; (e) influence of ions (10 mM each) on PLA₂ activity in the absence or presence of 1 mM Ca²⁺; (f) effect of substrate concentration on the kinetics of BbilTX (PLA₂) activity. The inset shows the curve shape at low substrate concentrations. The results of all experiments are the mean ± SE, of three determinations (P < 0.05).