Table 1.
Sequence obtained by MS/MS based on the alkylated tryptic peptides derived. The peptides were separated and sequenced by mass spectrometry.
| Residue number | Mass (Da) expected | Amino acid sequence | Mass (Da) calculated |
|---|---|---|---|
| 1–7 | 898.5293 | HLLQFNK | 898.5025 |
| 16–33 | 2157.9352 | NAIPFYAFYGCYCGWGGR | 2157.9189 |
| 43–53 | 1504.5356 | CCFVHDCCYGK | 1504.5356 |
| 61–69 | 1183.6216 | WDIYPYSLK | 1183.5913 |
| 70–77 | 884.4277 | SGYITCGK | 884.4062 |
| 78–90 | 871.8404 | GTWCEEQICECDR | 1741.6494 |
| 91–98 | 973.5307 | VAAECLRR | 973.5127 |
| 98–104 | 853.4780 | RSLSTYK | 853.4657 |
| 105–114 | 1297.5059 | YGYMFYPDSR | 1297.5437 |
| 115–122 | 965.3514 | CRGPSETC | 965.3695 |