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Dynamic range, linearity, specificity, and reproducibility. QPCR amplification of 7-point standard curves from 3 × 107 to 30 copies of (a) CO2, (c) D-loop, and (e) calicin markers. Melting curves showing the negative first derivative of the fluorescence signal of (b) CO2, (d) D-loop, and (f) calicin PCR product.
