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Experimental schemes. (a) The distribution of PS from microplates that were preincubated with PSs and then incubated with A431 cells was analyzed in three microplates (MP 1, MP 2, and MP 3). (b) Different incubation protocols were applied prior to photodynamic treatment to assess the phototoxicity of microplate-adhered PS. Abbreviations: DPBS: Dulbecco's phosphate-buffered saline; FBS: fetal bovine serum; MP: microplate; PS: photosensitizer; SN: supernatant.
