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. 2013 Mar 7;9(3):e1002934. doi: 10.1371/journal.pcbi.1002934

Figure 1. Illustration of the master strain and library construction procedure.

Figure 1

A master strain was constructed such that it will contain two main constructs in the HIS deletion locus: a constant control construct with mCherry driven by the TEF2 promoter and terminated by a constant ADH1 terminator; and a test construct with a YFP gene driven by Gal1/10 promoter. Following master strain construction, a library of PCR products containing the downstream intergenic regions of 85 tested genes was amplified from the genome by PCR and extended to also contain the URA3 promoter and start codon. This library of DNA sequences was then integrated into the master strain such that only integrations in the exact genomic location would result in an intact selection marker.