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. 2013 Mar 7;9(3):e1003324. doi: 10.1371/journal.pgen.1003324

Figure 4. Constitutive repressor function of atf-7(gf) mutation blocks tph-1::gfp upregulation induced by tir-1(gf) and PA14.

Figure 4

A. Quantification of ADF tph-1::gfp in atf-7(lf), atf-7(gf) and atf-7(gf) carrying the Ex[tir-1(yz68gf)] transgene under optimal growth conditions, on their own or in the background of ocr-2 mutation. B. atf-7(lf), atf-7(gf) and daf-19 mutants failed to upregulate ADF tph-1::gfp following 6 hr incubation on a lawn of PA14 relative to their sibling incubated on OP50. One-day-old adults were tested. C. atf-7(lf) and atf-7(gf) mutations, on their own or in ocr-2 mutation background, did not prevent ADF tph-1::gfp upregulation during dauer formation, as compared to L4 worms. Data represent the average of three trials each with at least 20 animals per strain per condition ± SEM. The value of GFP fluorescence in the ADF neurons of WT animals under a stress paradigm and that of mutants is normalized to the value of WT animals under optimal conditions. ** p<0.01, *** p<0.001.