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. 2013 Mar 7;9(3):e1003324. doi: 10.1371/journal.pgen.1003324

Figure 6. Reduced immune gene marker in the intestine of daf-19 mutants.

Figure 6

A–H. Representative photomicrographs showing two-day-old adult WT and indicated mutants expressing a GFP reporter for the ShK-like toxin peptide gene T24B8.5 (T24B8.5::gfp) in the intestine, all the animals shown with the anterior to the left. I. Quantification of T24B8.5::gfp intensity in the intestine of two-day-old adults. Fluorescence was quantified by measuring pixel intensity of three areas along the body (as depicted in B). T24B8.5::gfp intensity was reduced in atf-7(gf) and two daf-19 alleles, but increased in tir-1(yz68gf) and atf-7(lf) mutants. daf-19 deficiency diminished the increased T24B8.5::gfp in tir-1(yz68gf) as well as atf-7(lf) mutants. Data represent the average of three trials each with 20 animals per strain ± SEM, and the value of GFP fluorescence in mutants is normalized to that of WT animals analyzed on the same day. Statistics between WT and mutants is marked on the top of each bar, and that between two indicated groups is marked on the top of lines, * p<0.05, ** p<0.01, *** p<0.001.