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. 2013 Mar 7;9(3):e1003335. doi: 10.1371/journal.pgen.1003335

Figure 2. SUN-1 phosphorylation is prolonged in the absence of DSBs and can be decreased to wild-type length by low-dosage irradiation.

Figure 2

WT (left) and spo-11(ok79) (right) hermaphrodite gonad, not irradiated (top) or irradiated with 7.5 Gy (bottom) dissected 8 h post-irradiation and stained with DAPI (top, blue in merge) and anti-SUN-1 S8Pi (bottom, green in merge). Schematics on the bottom of each gonad represent nuclei with and without phosphorylation of SUN-1 (S8, S12, S24, and S43) in the meiotic part of the gonad (from meiotic prophase entry to beginning of cellularization/diplotene in WT, marked with dotted lines in DAPI channels). Orange represents cell rows with ≥50% of nuclei with SUN-1 phosphorylation.