Table 2. Plasmids and strains used and the associated literature source.
Strains/plasmid | Description | Source/Reference |
E.coli strain M15pREP4 | Expression host containing pREP4; kanamycin resistant | Qiagen |
pQE30 | IPTG inducible expression vector. Introduces RGS(H)6 at the N terminus of the expressed protein. Confers ampicillin resistance | Qiagen |
pQE60 | IPTG inducible expression vector. Introduces RGS(H)6 at the C terminus of the expressed protein. Confers ampicillin resistance | Qiagen |
pQE60A3P1 | CheA3P1 expression plasmid. pQE60 derivative | [7] |
pQEY6 | CheY6 expression plasmid. pQE30 derivative | [24] |
pQEA3 | CheA3 expression plasmid. pQE30 derivative | [21] |
pQEA4 | CheA4 expression plasmid. pQE30 derivative | [21] |