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. 2013 Mar 7;9(3):e1003213. doi: 10.1371/journal.ppat.1003213

Figure 1. Only a basic set of Rab-GTPases is present in apicomplexan parasites.

Figure 1

(A) Unrooted neighbour joining tree of the GTPase domains identified from each conserved Rab sequence of the eight canonical species and T.gondii. Clades containing sequences derived from T. gondii are indicated by shaded wedges. Three major clades containing T. gondii sequences are evident containing sequences of a) Rab1 and18 b) Rab5,6,7 and 23 c) Rab2,4 and 11. The species origin of the nodes is shown by a colour code as indicated. For detailed subtrees of major clades and support values see Figure S2). (B) Immunofluorescence analysis of intracellular parasites expressing ddFKBPmyc-Rab1A,B,2,4,5A,5C,7 and 18 or Rab5B-ddFKBPHA-construct treated for 18 hrs with 1 µM Shld-1 prior to fixation. The left panel shows Rabs costained with ER/Golgi marker (TgERD2 and TgGalNAC in green), whereas the right panel shows co-staining with markers for ELCs (TgVP1 and proM2AP in red), the nucleus is shown in blue (Dapi). The scale bars represent 5 µm. 1 =  Nucleus, 2 =  Apicoplast, 3 =  Mitochondrium, 4 =  Dense Granule, 5 =  Endoplasmatic Reticulum (ER), 6 =  Golgi, 7 =  late endosome-like compartment (TgVP1 compartment), 8 =  early endosome like compartment (proM2AP), 9 =  Micronemes, 10 =  Rhoptries. See also Figure S4&S5 for more details.