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. 2013 Mar 7;9(3):e1003202. doi: 10.1371/journal.ppat.1003202

Figure 4. 2H10, bi-2H10 and bi-2H10 W100A do not interact with membrane anchored Env.

Figure 4

FACS analysis of HEK293T cells transfected with JR-FL Env in its cleaved (A) and un-cleaved form (B). The increase in mean fluorescence intensity (MFI) has been blotted as a function of antibody concentration. Control antibodies include b12, b6, b12-Bio (biotinylated version of B12) and 4E10. The lack of significant b6 interaction with the cells expressing cleaved Env indicates that most Env has been processed.