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. 2013 Mar 7;8(3):e58405. doi: 10.1371/journal.pone.0058405

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© 2013 Hebert et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Pitx2 (green) and dystrophin (red) localization in wild-type mouse EOM. Arrows point down to Pitx2-positive myonuclei. Arrowheads point up to Pitx2-positive nuclei outside of the dystrophin ring. (C) Pitx2 (green) and laminin (red) localization in wild-type mouse EOM. (D) Pitx2 (green) and dystrophin (red) localization in wild-type mouse TA. Arrow points to a rare Pitx2-positive cell. (E) Pitx2 (green) and dystrophin (red) localization in human EOM. (F) Pitx2 (green) and dystrophin (red) localization in aged (19 months old) wild-type mouse EOM. (G) Percentages of Pitx2-positive nuclei in relation to dystrophin in wild-type mouse EOM and TA. (H) Percentages of Pitx2-positive nuclei in relation to laminin in wild-type mouse EOM and TA. orb, orbital layer of EOM. glob, global layer of EOM. Scale bars are 50 µm. * Indicates significant difference from EOM orbital inside (P≤0.05). # Indicates significant difference from EOM global inside (P≤0.05).