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. 2013 Mar 7;8(3):e58405. doi: 10.1371/journal.pone.0058405

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© 2013 Hebert et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Pitx2 immunostaining of cytospun EECD34 cells from wild-type mouse EOM and TA. (C) Relative transcript levels of Pitx2 in cultured EECD34 cells from wild-type mouse EOM and TA. (D) Percentage of freshly isolated EECD34 cells from wild-type mouse EOM and TA that are Pitx2-positive determined by immunostaining of cytospun cells. (E) Percentage of freshly isolated EECD34 cells from wild-type mouse EOM and TA that are Pitx2-positive determined by flow cytometry. (F) Percentage of all freshly isolated mononuclear cells from wild-type mouse EOM and TA that are Pitx2-positive EECD34 cells determined by flow cytometry. Scale bars are 50 µm. * Indicates significant difference from EOM (P≤0.05).